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Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

DOI:
10.3791/51151-v

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09:27 min

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January 17, 2014

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, , , , ,
1School of Molecular Bioscience,University of Sydney, 2Department of Medicine,Center for Vascular Research, 3Asia Pacific Centre for Animal Health, Faculty of Veterinary Science,University of Melbourne

Chapters

  • 00:05Title
  • 01:34Creating the Recombinant Vector
  • 02:48Recombinant Virus Generation
  • 05:26Plaque Assay: NO Selection
  • 05:59Purity of Viral Stocks: PCR Screening of Genomic DNA
  • 07:23Results: Recombinant Vectors and the VACV Genome
  • 08:43Conclusion

Summary

자동 번역

자동 번역

A rapid and modular protocol for the generation of recombinant vaccinia viruses expressing fluorescently tagged proteins simultaneously using the method of transient dominant selection is described here.

Tags

Fluorescent Protein TaggingVaccinia VirusRecombinant Virus GenerationHomologous RecombinationTransient Dominant SelectionMetabolic SelectionLive-cell MicroscopyVirus-host Interactions

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