Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Rapid Synthesis and Screening of Chemically…
JoVE Journal
Biology

이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.

JoVE Journal Biology
Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
 

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Article DOI: 10.3791/51153-v • 09:22 min • November 26th, 2013
November 26th, 2013 •
*1,3, *1, 1,2, 1
1The Lewis-Sigler Institute for Integrative Genomics, Princeton University, 2Department of Molecular Biology, Princeton University, 3Division of Chemistry and Chemical Engineering, California Institute of Technology
* These authors contributed equally

챕터

요약
Automatic Translation

This protocol describes an experimental procedure for the rapid construction of artificial transcription factors (ATFs) with cognate GFP reporters and quantification of the ATFs ability to stimulate GFP expression via flow cytometry.

Tags

Rapid Synthesis Screening Chemically Activated Transcription Factors GFP-based Reporters Synthetic Biology Gene Expression Synthetic Circuits Quantitative Properties Native Control Circuits Gene Expression Programming Off-target Effects Yeast Strains ACT1 Promoter URA3 Cassette Ligand-binding Domain Human Estrogen Receptor VP16 Linear PCR Product DNA Binding Domain Constitutively Expressed Artificial Transcription Factor (ATF) Homologous Recombination Inducer Off-target Activation Nonphysiological Growth Conditions Conditional Gene Expression Systems GFP Reporter Plasmids
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter