Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection

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신경과학

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DOI:

10.3791/51168-v

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11:46 min

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January 13, 2014

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Urmi Bandyopadhyay², Wayne A. Fenton, Arthur L. Horwich², Maria Nagy²

¹Department of Genetics,Yale School of Medicine, ²Howard Hughes Medical Institute

Chapters

00:05Title
02:23Preparing Spinal Cord Sections
04:21Staining Spinal Cord Sections
07:20Laser Capture Microdissection (LMD)
10:08Results: Motor Neuron Isolation
11:07Conclusion

Summary

자동 번역

High-quality total RNA has been prepared from cell bodies of mouse spinal cord motor neurons by laser capture microdissection after staining spinal cord sections with Azure B in 70% ethanol. Sufficient RNA (~40-60 ng) is recovered from 3,000-4,000 motor neurons to allow downstream RNA analysis by RNA-seq and qRT-PCR.

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Laser Capture Microdissection of Drosophila Peripheral Neurons

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling

Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection

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Chapters

Summary

자동 번역

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Article

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