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FIBS-enabled Noninvasive metabole
JoVE 신문
생체공학
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JoVE 신문 생체공학
FIBS-enabled Noninvasive Metabolic Profiling
DOI:

09:16 min

February 03, 2014

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Chapters

  • 00:05Title
  • 01:18Cell Line Revival and Maintenance
  • 02:06Cell Line Transfection with the Biosensor Gene-containing Plasmid
  • 03:47Batch and Fed-batch Cell growth Curve
  • 04:44FRET Ratio Measurements
  • 05:36Metabolite Assays
  • 06:08Results: Representative Fed-batch Culture and Corresponding FRET Measurements
  • 08:54Conclusion

Summary

자동 번역

Een beschrijving van hoe je Förster Resonance Energy Transfer geïntegreerde biologische sensoren (FIBS) kalibreren voor in situ metabool profiel wordt gepresenteerd. De FIBS kan worden gebruikt om de intracellulaire niveaus van metabolieten invasief leveren aan de ontwikkeling van metabole modellen en hoge doorvoer screening van bioproces omstandigheden te meten.

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