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人类神经的聚集体cGMP的适用展开法干细胞和祖细胞来源从多能干细胞或胎儿脑组织

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A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue

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人类神经的聚集体cGMP的适用展开法干细胞和祖细胞来源从多能干细胞或胎儿脑组织

DOI:

10.3791/51219-v

•

09:37 min

•

June 15, 2014

•

Brandon C. Shelley, Geneviève Gowing, Clive N. Svendsen

¹Regenerative Medicine Institute,Cedars-Sinai Medical Center

Chapters

00:05Title
01:36hNPC Observations
02:13Chopper Setup
03:22Pre-chop Procedure
05:58Chop Procedure
07:08Post-chop Procedure
08:11Representative Results of hNPCs Aggregates Using a cGMP-applicable Expansion Method
09:04Conclusion

Summary

자동 번역

这个协议描述了一种新颖的机械斩波方法，它允许球形神经干细胞和祖细胞的聚集体的膨胀而不分离为单个细胞悬浮液。维持细胞/细胞接触可超过40代快速稳定增长。

Tags

CGMP Expansion Method Neural Stem Cells Neural Progenitor Cells Pluripotent Stem Cells Neurospheres Cell Culture Mechanical Passaging Automated Chopping Suspension Culture Cell Yield Scale-up Clinical-grade Cell Production

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