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One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice
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One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice

One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice

DOI:
10.3791/51225-v

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09:52 min

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February 25, 2014

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1Center for Regenerative Therapies Dresden,Technische Universität Dresden, 2German Center for Neurodegenerative Diseases (DZNE) Dresden

Chapters

  • 00:05Title
  • 01:41Microdissection of Mouse Brain
  • 04:13SVZ Tissue Dissociation
  • 05:43DG Tissue Dissociation
  • 07:36Cell Plating for Monolayer and Neurosphere Culture Generation
  • 08:17Results: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual Mice
  • 09:21Conclusion

Summary

자동 번역

자동 번역

Here we describe a detailed protocol for the simultaneous generation of neural precursor cell cultures, as either adherent monolayers or neurospheres, from the subventricular zone and dentate gyrus of individual adult mice.

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Keywords: Neural Stem CellsNeurosphere AssayAdherent Monolayer CultureAdult NeurogenesisSubventricular ZoneDentate GyrusCell ProliferationCell DifferentiationMouse Models

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