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凝血因子VIII的脂质纳米管螺旋组织

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Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes

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凝血因子VIII的脂质纳米管螺旋组织

DOI:

10.3791/51254-v

•

12:24 min

•

June 03, 2014

•

Jaimy Miller*¹, Daniela Dalm*¹, Alexey Y. Koyfman³, Kirill Grushin, Svetla Stoilova-McPhie³

¹Department of Neuroscience and Cell Biology,University of Texas Medical Branch, ²Department of Biochemistry and Molecular Biology,University of Texas Medical Branch, ³Sealy Center for Structural Biology and Molecular Biophysics,University of Texas Medical Branch

Chapters

00:05Title
01:21Cryo-electron Microscopy of Factor VIII Bound to Lipid Nanotubes
07:193D Reconstruction of Porcine Factor VIII Bound to Lipid Nanotubes
08:21Electron Tomography of Porcine Factor VIII Bound to Lipid Nanotubes
09:42Results: Factor VIII Helical Organization on Lipid Nanotubes
11:42Conclusion

Summary

자동 번역

我们提出了低温电子显微镜，脂质纳米技术和结构分析的组合应用，以解决两个高度同源的FVIII形式与膜结合的结构：人和猪。在我们研制的实验室，以螺旋状组织两个功能重组FVIII形式对带负电荷的脂质纳米管（LNT）的方法进行说明。

Tags

Keywords: Cryo-electron Microscopy Factor VIII Blood Coagulation Lipid Nanotubes Hemophilia Protein Structure Recombinant FVIII Electron Tomography 3D Reconstruction

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