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Chapters
- 00:05Title
- 00:54Preparation of Staples Pool and Fabrication Reaction Mixtures
- 02:30Temperature Annealing Ramp for Fabrication Reaction and Removal of Excess Staples
- 04:07Agarose Gel Electrophoresis Analysis and Negative Stain with Uranyl-formate
- 06:10Results: Bio-responsive Robot Purity and Yield Analysis
- 07:33Conclusion
DNA origami is a powerful method for fabricating precise nanoscale objects by programming the self-assembly of DNA molecules. Here we describe a protocol for the folding of a bio-responsive robot from DNA origami, its purification and negative staining for transmission electron microscopic imaging (TEM).
