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Immunology and Infection

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JoVE Journal Immunology and Infection
Activering en waardering van NLRP3 inflammasome activiteit met behulp van IL-1β in Human Monocyt-afgeleide dendritische cellen
 
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Activering en waardering van NLRP3 inflammasome activiteit met behulp van IL-1β in Human Monocyt-afgeleide dendritische cellen

Article DOI: 10.3791/51284-v • 09:04 min • May 22nd, 2014
May 22nd, 2014 •
1, 2, 3
1Department of Pathology, New York University School of Medicine, 2Division of Infectious Diseases, Department of Medicine, Mount Sinai Medical Center, 3Division of Hematology and Oncology, Hess Center for Science and Medicine, Mount Sinai Medical Center

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Dendritische cellen (DCs) scheiden IL-1β reactie op TLR8 erkenning van synthetische purine, R848, gevolgd door NLRP3 inflammasome activering met nigericine derhalve IL-1β kunnen worden gebruikt om NLRP3 inflammasome te meten. Intracellulaire cytokine kleuring, immunoblotting en ELISA worden gebruikt om NLRP3 inflammasome priming en activering van IL-1β expressie nauwkeurig te meten.

Tags

Immunologie NLRP3 inflammasome IL-1 beta interleukine-1 bèta dendritische cel nigericine Toll-like receptor 8 TLR8 R848 monocyten afgeleide dendritische cellen
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