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Expressie, isolatie en zuivering van oplosbare en onoplosbare Biotinylated Eiwitten voor Nerve Tissue Regeneration
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JoVE 신문 생체공학
Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration
DOI:

12:03 min

January 22, 2014

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Chapters

  • 00:05Title
  • 02:55Bacterial Cell Culture
  • 04:25Isolation and Purification of Recombinant Protein
  • 05:42Ni-NTA Affinity Chromatography
  • 08:31Results: Purification of Native and Nonnative Proteins for Biotinylation
  • 10:32Conclusion

Summary

자동 번역

Ontwikkelen biotinylatable fusie-eiwitten heeft veel potentiële toepassingen in diverse gebieden van onderzoek. Recombinant eiwit-engineering is een rechttoe rechtaan procedure die is kosteneffectief, het verstrekken van hoge opbrengsten van op maat gemaakte eiwitten.

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