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使用无标记光学生物传感器由G-蛋白偶联受体,以检测钾通道的调制
JoVE 신문
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JoVE 신문 생체공학
Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
DOI:

10:59 min

February 10, 2014

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Chapters

  • 00:05Title
  • 02:30384-well Optical Biosensor Plate Coating
  • 03:39Seeding of Cells onto the 384-well Optical Biosensor Plate
  • 04:46Resonance Wavelength Grating (RWG) Optical Biosensor Assay
  • 06:40Analysis of Assay Data with BIND View
  • 07:52Results: Analysis of Peak Wavelength Value to Detect Modulation of Potassium Channels
  • 10:02Conclusion

Summary

자동 번역

光学生物传感器技术可检测的变化在质量近质膜在活细胞中,并允许1到跟随在这两个单个细胞和细胞群的细胞反应。这个协议将描述检测钾通道通过使用这种方法的G蛋白偶联受体在完整的细胞调制的。

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