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Chapters
- 00:05Title
- 01:21Preparation
- 02:36Pial Surface Plasmid Injection
- 03:38Electroporation
- 05:27Results: Pial Surface Expression
- 05:58Conclusion
The pial surface is a unique progenitor zone in the CNS that is receiving increasing attention. Herein, we detail a method for rapid genetic manipulation of this progenitor zone using a modified electroporation method. This procedure can be used for cellular and molecular investigations of cell lineages and signaling pathways involved in cell differentiation and to elucidate the fate and properties of daughter cells.
