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快速，特异微孔板检测为内和细胞外抗坏血酸在培养细胞的测定

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A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

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快速，特异微孔板检测为内和细胞外抗坏血酸在培养细胞的测定

DOI:

10.3791/51322-v

•

11:56 min

•

April 11, 2014

•

Darius J. R. Lane, Alfons Lawen

¹Molecular Pharmacology and Pathology Program, Department of Pathology & Bosch Institute,University of Sydney, ²Department of Biochemistry and Molecular Biology, School of Biomedical Sciences,Monash University

Chapters

00:05Title
02:01Determine Intracellular Ascorbate in Cultured Cells
04:16Determine the Amount of Ascorbate Released and Extracellular Ascorbate
06:25Results: Determination of Intracellular and Extracellular Ascorbate in Cultured Cells
11:36Conclusion

Summary

자동 번역

抗坏血酸发挥许多重要作用的细胞代谢，其中许多只明朗化的趋势。这里，我们描述一个介质通量，为在细胞培养物内和细胞外抗坏血酸的确定具体且廉价微孔板检测。

Tags

Keywords: Ascorbate Vitamin C Microplate Assay Intracellular Extracellular Cell Culture Medium-throughput High-sensitivity Iron Metabolism Neuroprotective Neuromodulatory Brain Homeostasis

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