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在斑马鱼胚胎氧化应激分析
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Analysis of Oxidative Stress in Zebrafish Embryos
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在斑马鱼胚胎氧化应激分析

DOI:
10.3791/51328-v

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11:05 min

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July 07, 2014

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, ,
1Department of Molecular Biotechnology and Health Science,University of Torino, 2Laboratory of Endothelial Molecular Biology,Vesalius Research Center, VIB

Chapters

  • 00:05Title
  • 01:54Preparations for Oxidative Stress Induction and ROS-detection
  • 03:01Oxidative Stress Induction
  • 04:28Whole Mount ROS-detection
  • 05:56Single Cell ROS-detection
  • 09:08ROS-detection in Whole Mount and Single Cells
  • 10:30Conclusion

Summary

자동 번역

자동 번역

Here we report a protocol to measure oxidative stress in living zebrafish embryos. This procedure allows reactive oxygen species (ROS) detection in both whole embryo tissues and single-cell populations. This protocol will accomplish both qualitative and quantitative analyses.

Tags

Keywords: Oxidative StressReactive Oxygen SpeciesROSZebrafish EmbryosIn VivoFluorescent ROS ProbesTransgenic Fluorescent LinesWhole Embryo ROS-detectionSingle-cell ROS DetectionOxidant AgentsGenetic ScreensAnimal ModelsNeurological DisordersCancer

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