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면역학 및 감염병학

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检测荧光纳米粒子的相互作用与原发性免疫细胞亚群流式细胞仪的

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry

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检测荧光纳米粒子的相互作用与原发性免疫细胞亚群流式细胞仪的

DOI:

10.3791/51345-v

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07:31 min

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March 28, 2014

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Olimpia Gamucci, Alice Bertero², Maria Ada Malvindi, Stefania Sabella, Pier Paolo Pompa, Barbara Mazzolai, Giuseppe Bardi

¹Center for Micro-BioRobotics @SSSA,Istituto Italiano di Tecnologia, ²Department of Biology,University of Pisa, ³Center for Biomolecular Nanotechnologies @UniLe,Istituto Italiano di Tecnologia

Chapters

00:05Title
00:56Nanoparticle Internalization in Blood Leukocytes and Purified Monocytes
02:22Flow Cytometric Analysis
03:44Results: Representative FITC-SiO₂ Nanoparticle Internalization Analyses
06:55Conclusion

Summary

자동 번역

分析与免疫细胞的流式细胞仪检测亚组人群纳米粒子的相互作用。

Tags

Flow Cytometry Nanoparticle Interaction Immune Cells FITC-SiO2 Nanoparticles Rhodamine-SiO2 Nanoparticles Primary Microglia GFP-expressing Monocyte/macrophage Lineage Cell Fluorescence Side-scattered Light

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