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在伴有海藻酸钠生产新基因的鉴定<em>铜绿假单胞菌</em>使用小型<em> himar1</em>水手转座子介导的诱变
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis
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在伴有海藻酸钠生产新基因的鉴定<em>铜绿假单胞菌</em>使用小型<em> himar1</em>水手转座子介导的诱变

DOI:
10.3791/51346-v

•

08:57 min

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March 10, 2014

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, ,
1Department of Biochemistry and Microbiology, Joan C. Edwards School of Medicine,Marshall University

Chapters

  • 00:05Title
  • 01:42Preparation of Bacterial Strains and Biparental Conjugation
  • 04:42Detection and Isolation of Mucoid Colonies
  • 06:00gDNA Restriction Digestion and Ligation and Inverse PCR and Sequence Analysis
  • 07:07Results: Mini-himar1 Mariner Transposon-mediated Mutagenesis
  • 08:28Conclusion

Summary

자동 번역

자동 번역

这里我们描述了使用微型himar1水手转座子介导的诱变以产生高密度的插入突变体文库，以屏幕的协议，分离和鉴定中的原型的铜绿假单胞菌菌株PAO1新颖藻稳压器。

Tags

Keywords: Pseudomonas AeruginosaAlginateMucoidyMini-himar1 TransposonMutagenesisMucEKinBAlgUMucACystic FibrosisBiofilmsVirulence GenesColony Morphology

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