JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:02Preparation of LC-MS Reagents and Establishment of a Chromatography Method and Instrument Operating Proedures
- 04:17Preparation of Metabolite Samples
- 06:11Setup of the Sample Sequence
- 07:07Analysis of LC-MS Data
- 09:51Results: Evaluation of Instrument Accuracy, Sensitivity, and Untargeted Metabolomics Based on Current LC-QE-MS Platform
- 13:47Conclusion
Metabolite profiling has been a valuable asset in the study of metabolism in health and disease. Utilizing normal-phased liquid chromatography coupled to high-resolution mass spectrometry with polarity switching and a rapid duty cycle, we describe a protocol to analyze the polar metabolic composition of biological material with high sensitivity, accuracy, and resolution.
