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与落射荧光组织基质成分和双光子显微镜的长期活体免疫荧光成像
JoVE 신문
생체공학
This content is Free Access.
JoVE 신문 생체공학
Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy
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与落射荧光组织基质成分和双光子显微镜的长期活体免疫荧光成像

DOI:
10.3791/51388-v

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09:00 min

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April 22, 2014

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, , , ,
1Institute of Bioengineering and Swiss Institute of Experimental Cancer Research (ISREC),École Polytechnique Fédérale de Lausanne, 2Department of Cell and Developmental Biology and Knight Cancer Institute,Oregon Health & Science University

Chapters

  • 00:05Title
  • 01:50Prepare Tumor Cells and Ear Injection
  • 03:36Ear Surgery
  • 04:40Immunofluorescence Staining and Intravital Imaging with a Stereomicroscope
  • 07:06Results: Intravital Immunofluorescence Imaging
  • 08:26Conclusion

Summary

자동 번역

자동 번역

细胞外基质在伤口愈合，炎症和肿瘤发生实质性的重塑。我们提出了一个新颖的活体免疫荧光显微镜方法来可视化纤维状的网状基质成分使用落射荧光或双光子显微镜的高空间和时间分辨率的动态以及。

Tags

Extracellular MatrixTumor MicroenvironmentMatrix RemodelingIntravital ImagingEpifluorescence MicroscopyTwo-photon MicroscopyCollagenTenascin CBasement MembraneTumor Cell MigrationT-cell Interaction

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