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Chapters
- 00:05Title
- 01:41Preparing Solutions and Cells
- 03:58Imaging the Cells
- 05:53Data Analysis
- 07:03Intracellular pH Changes in HT-1080 Cells
- 08:57Conclusion
Several methods are available for measuring intracellular pH but very few of these allow simultaneous measurement of cytoplasmic and organellar pH. Here, we describe in detail a rapid and accurate methodology to simultaneously measure cytoplasmic and vesicular pH by ratiometric imaging of living cells.
