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Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

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Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

DOI:

10.3791/51408-v

•

09:03 min

•

May 29, 2014

•

HoTae Lim, In Young Choi, Gabsang Lee

¹Institute for Cell Engineering, Department of Neurology and Neuroscience,Johns Hopkins University School of Medicine

Chapters

00:05Title
01:20Preparing hESCs for FACS Purification
02:26Lysis of FACS-purified Single Cell in Each Well of a 96-well Plate
03:28Reverse Transcription
04:29Amplification
05:26qRT-PCR Performance
06:21Results: qRT-PCR is an Efficient Method for Profiling Single Cell Gene Expression
08:39Conclusion

Summary

자동 번역

Single cell gene expression assay is needed for understanding stem cell heterogeneities.

Tags

Single Cell Transcriptome QRT-PCR Heterogeneity Stem Cell Differentiation OCT4 NANOG Gene Expression

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