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人中性粒细胞流动室粘附实验
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Human Neutrophil Flow Chamber Adhesion Assay
DOI:

12:42 min

July 02, 2014

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Chapters

  • 00:05Title
  • 01:50HUVEC Layer Preparation
  • 03:07Purified Ligand Coating
  • 05:11Neutrophil Separation
  • 08:33Flow Chamber Adhesion Assay
  • 10:20Results: The Flow Chamber Assay Enables Analyses of Adhesive Properties of Human Peripheral Blood Neutrophils to HUVECs and Purified Ligand Substrates
  • 11:49Conclusion

Summary

자동 번역

报道定量中性粒细胞粘附的方法。此方法创建类似于血管遇到了动态流环境。它使中性粒细胞粘附到任何纯化黏附分子(配体)或类似体内环境与剪切应力的上下文内皮细胞基板(HUVEC)的调查。

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