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Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
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Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

DOI:
10.3791/51411-v

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12:04 min

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March 27, 2014

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,
1Forage-Animal Production Research Unit, Agricultural Research Service,United States Department of Agriculture

Chapters

  • 00:05Title
  • 01:22Preparation of Thin-layer Plates
  • 03:39Preparation of Developing Chambers for Extract Separation
  • 04:23Loading and Development of TLC Plates
  • 06:04Preparation of Plates for Bioassay
  • 07:41Bacterial Culture and Assay
  • 09:14Visualization of Bioassayed Agar Plates
  • 10:07TLC and Contact Bioautography Assay Results
  • 11:21Conclusion

Summary

자동 번역

자동 번역

Methods are described for thin-layer chromatographic (TLC) separation of plant extracts and contact bioautography to identify antibacterial metabolites. The methods are applied to the screening of red clover phenolic compounds inhibiting hyper ammonia-producing bacteria (HAB) native to the bovine rumen.

Tags

Keywords: Thin-layer ChromatographyTLCBioassayPlant ExtractAntimicrobial CompoundsBioautographyMicrobial GrowthUV VisualizationTetrazolium Red StainingClostridium SticklandiiRed CloverPhenolic CompoundsHyper Ammonia-producing BacteriaRumen

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