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면역학 및 감염병학

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在抗病毒模式识别受体RIG-I和PKR通过有限蛋白酶消化和Native PAGE监测激活

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE

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在抗病毒模式识别受体RIG-I和PKR通过有限蛋白酶消化和Native PAGE监测激活

DOI:

10.3791/51415-v

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12:43 min

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July 29, 2014

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Michaela Weber, Friedemann Weber

¹Institute for Virology,Philipps-University Marburg

Chapters

00:05Title
02:25Infection of A549 Cells with Rift Valley Fever Virus Clone 13 (Cl 13)
04:17Preparation od Cell Lysates
05:47Determination of Conformational Changes of Pattern Recognition Receptors
08:40Analysis of Oligomeric States of Pattern Recognition Receptors
09:46Results: RIG-I and PKR Assay
12:00Conclusion

Summary

자동 번역

先天防御病毒感染是由模式识别受体（模式识别受体）触发。这两个细胞质模式识别受体RIG-I和PKR结合到病毒签名的RNA，构象变化，低聚，并激活抗病毒信号。方法被描述，其允许方便地监控构象交换和这些细胞质的PRR的低聚。

Tags

Pattern Recognition Receptors RIG-I PKR Antiviral Response Protease Digestion Native PAGE Host Defenses Viral RNA Ligands Interferon Response Gene Expression Trypsin Digestion Protease Resistance Phosphorylation Oligomeric Complexes

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