Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis

00:05Title
01:30Preparation of Antibody Coated Magnetic Beads and Extraction of Nuclei from Drosophila Larval Tissues
04:16Collection of Pre-isolation Samples
06:16Isolation of GFP-tagged Nuclei from Drosophila Larval Tissues
08:45Results: Analysis of Tagged-nuclei Enrichment “Pre-isolatoin” and “Post-isolation” Samples
12:28Conclusion

Summary

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Drosophila væv ofte indeholder en heterogen blanding af celletyper. For at undersøge genekspression i specifikke celletyper fra et bestemt væv, kan kerner genetisk mærkede og efterfølgende isoleret ved anvendelse af en affinitet tilgang. Isolerede kerner kan anvendes til efterfølgende anvendelser, såsom genekspression analyse og kromatin immunofældning.

Affinity-baserede Isolering af Tagged Kerner fra<em> Drosophila</em> væv til genekspressionsanalyse

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