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MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules
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JoVE 신문 생물학
MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in Medicago truncatula Root Nodules
DOI:

09:59 min

March 05, 2014

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Chapters

  • 00:05Title
  • 01:12Tissue Preparation
  • 02:27Matrix Application
  • 04:28Image Acquisition, Image Generation, and Metabolite Identification
  • 06:28Results: Discovery and Identification of Small Molecule Analytes in Intact Tissue by MALDI-MSI
  • 09:28Conclusion

Summary

자동 번역

Mass spectrometric imaging (MSI) is a powerful tool that can be used to discover and identify various chemical species in intact tissues, preserving the compounds in their native environments, which can provide new insights into biological processes. Herein a MSI method developed for the analysis of small molecules is described. 

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