Journal
/
/
Сочетание Манипуляции одиночных молекул и визуализации по изучению взаимодействий белок-ДНК
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
DOI:

14:43 min

August 27, 2014

, , , ,

Chapters

  • 00:05Title
  • 02:39Optical Tweezers Setup with Nanometer Stability
  • 04:40Microfluidics
  • 08:36Combined Optical Trapping and Single-molecule Fluorescence Imaging Experiments
  • 11:50Results: Detection of Single Protein Molecules Interacting with a Single DNA Molecule
  • 12:56Conclusion

Summary

자동 번역

Here we describe the instrumentation and methods for detecting single fluorescently-labeled protein molecules interacting with a single DNA molecule suspended between two optically trapped microspheres.

Related Videos

Read Article