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Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts
 

Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts

Article DOI: 10.3791/51465-v 07:13 min May 13th, 2014
May 13th, 2014

챕터

요약

Treatment of primary mouse osteoblasts with retinoic acid produces a homogeneous population of ramified cells bearing morphological and molecular features of osteocytes. The method overcomes the difficulty of obtaining and maintaining primary osteocytes in culture, and can be advantageous to study cells derived from transgenic models. 

Tags

Osteocyte Cultures Primary Mouse Osteoblasts Retinoic Acid Osteocyte Differentiation Osteoblast Markers Osteocyte-specific Molecules Transgenic Models Extracellular Matrix
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