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维甲酸的应用获取从初级小鼠成骨细胞骨细胞培养

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Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts

Please note that all translations are automatically generated. Click here for the English version.

维甲酸的应用获取从初级小鼠成骨细胞骨细胞培养

DOI:

10.3791/51465-v

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07:13 min

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May 13, 2014

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Deborah Mattinzoli, Piergiorgio Messa, Alessandro Corbelli, Masami Ikehata, Anna Mondini, Cristina Zennaro, Silvia Armelloni, Min Li, Laura Giardino, Maria Pia Rastaldi

¹Renal Research Laboratory,Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, ²Department of Nephrology, Dialysis and Renal Transplant,Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, ³Renal Physiopathology Laboratory, Department of Medical, Surgical and Health Sciences,University of Trieste

Chapters

00:05Title
01:46Isolation of Primary Osteoblasts
03:36All-trans Retinoic Acid Protocol
05:05Results: Osteocytes Generation using Retinoic Acid
06:31Conclusion

Summary

자동 번역

原代小鼠成骨细胞与维甲酸治疗产生分枝细胞轴承骨细胞的形态学和分子特征的同质人口。该方法克服了获得和保持主骨细胞在培养的难度，并且可以是有利的，研究从转基因模型中的细胞。

Tags

Osteocyte Cultures Primary Mouse Osteoblasts Retinoic Acid Osteocyte Differentiation Osteoblast Markers Osteocyte-specific Molecules Transgenic Models Extracellular Matrix

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