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Chapters
- 00:05Title
- 01:32Establishment of Primary Fibroblast Cultures from Colonic Explants
- 03:18Organotypic Preparation
- 06:52Organotypic Fixation and Laser Microdissection of the Invasive Margin
- 08:47Results: MicroRNA Expression Profiling of Tissue from Laser-microdissected Organotypic CRC Models
- 10:06Conclusion
Molecular profiling of laser microdissected cells and stroma from synthetic, tissue-engineered colorectal cancer models represents a novel and manipulatable approach to characterize and dissect the distinctive biology at the interface between tumor cells at the invasive front and cancer associated stromal cells.
