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Molecular Profiling van de invasieve tumor micro in een 3-dimensionaal model van colorectale kanker cellen en<em> Ex vivo</em> Fibroblasts
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JoVE 신문 의학
Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
DOI:

10:33 min

April 29, 2014

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Chapters

  • 00:05Title
  • 01:32Establishment of Primary Fibroblast Cultures from Colonic Explants
  • 03:18Organotypic Preparation
  • 06:52Organotypic Fixation and Laser Microdissection of the Invasive Margin
  • 08:47Results: MicroRNA Expression Profiling of Tissue from Laser-microdissected Organotypic CRC Models
  • 10:06Conclusion

Summary

자동 번역

Moleculaire profilering van laser gemicrodissecteerde cellen en stroma van synthetische, weefselengineering darmkanker modellen vertegenwoordigt een nieuwe benadering en manipuleerbaar te karakteriseren en ontleden de kenmerkende biologie op het grensvlak tussen tumorcellen aan de voorzijde en invasieve kanker geassocieerd stromacellen.

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