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Funzionalità di monitoraggio e di Morfologia Vasculature Reclutato da fattori secreti dalle cellule a crescita rapida, la generazione di tumore
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JoVE 신문 생물학
Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells
DOI:

09:03 min

November 23, 2014

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Chapters

  • 00:05Title
  • 01:29Extracellular Matrix (ECM)–mimic Gel Plug Inoculation into Mice
  • 02:43Evacuation of ECM–mimic Gel Plug Vascularization Using Ultrasound
  • 05:31Evacuation of ECM–mimic Gel Plug Vasculature Morphology Using Fibered Confocal Endomicroscopy
  • 07:23Results: Representative ECM–mimic Gel Plug Imaging
  • 08:40Conclusion

Summary

자동 번역

We describe a matrigel plug assay to illustrate angiogenic potential of a pool of factors secreted by cancer cells using two complementary imaging modalities, ultrasound and endomicroscopy. The matrigel, an extracellular matrix (ECM)-mimic gel, is utilized to introduce the host (mouse) with angiogenic factors secreted to the conditioned media (C.M.). 

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