Journal
/
생물학
/
Aip1p动力由R256H突变的肌动蛋白蚀变
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Aip1p Dynamics Are Altered by the R256H Mutation in Actin
Please note that all translations are automatically generated. Click here for the English version.

Aip1p动力由R256H突变的肌动蛋白蚀变

DOI:
10.3791/51551-v

•

08:57 min

•

July 30, 2014

•

, , ,
1Department of Pediatrics, Roy J. and Lucille A. Carver College of Medicine,University of Iowa, 2Department of Biochemistry, Roy J. and Lucille A. Carver College of Medicine,University of Iowa

Chapters

  • 00:04Title
  • 01:42Transforming the Plasmid into Yeast Cells
  • 03:36Visualizing Aip1p-GFP Protein Movement Using Total Internal Reflection Fluorescence Microscopy
  • 06:57Results: Quantification of Fluorescently Tagged Aip1p Movement in Wild Type and Mutant Cells
  • 08:29Conclusion

Summary

자동 번역

자동 번역

致病突变的肌动蛋白可以改变细胞骨架的功能。细胞骨架动力学是通过利用全内荧光显微镜荧光标记蛋白成像量化。作为一个例子，细胞骨架蛋白，Aip1p，已经改变了定位和运动在细胞中表达突变的肌动蛋白同种型，R256H。

Tags

ActinCytoskeletal DynamicsTIRF MicroscopyR256H MutationAip1pActin DepolymerizationLive Cell ImagingCytoskeletal FunctionRegulatory InteractionsProtein Function

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/51551?article_type=v

肌动蛋白共沉淀测定;​​ F -肌动蛋白结合蛋白分析
check_url/kr/51551?article_type=v

F - actin的动力学通过荧光活细胞成像斑点显微镜（密克罗尼西亚）
check_url/kr/51551?article_type=v

小动物的肌肉植入动态跟踪在体内荧光成像
check_url/kr/51551?article_type=v

漂白实验（FRAP和FLIP）来衡量生活胚胎干细胞的染色质的蛋白质动力学
check_url/kr/51551?article_type=v

表达GFP -肌动蛋白的内皮细胞的肌动蛋白细胞骨架的研究
check_url/kr/51551?article_type=v

从基因组DNA选择性捕获的5 - 羟甲基
check_url/kr/51551?article_type=v

在造血干细胞和祖细胞的成年小鼠颅骨骨髓体内四维跟踪
check_url/kr/51551?article_type=v

糖皮质激素粪分析：在马属动物无创监测肾上腺
check_url/kr/51551?article_type=v

高分辨率成像定时，并在活体人脐静脉内皮细胞微管动力学自动分析
check_url/kr/51551?article_type=v

三维超F-微丝高分辨率显微镜通过干涉光敏定位显微镜（iPALM）

Read Article