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Aip1p Dynamics ændres med R256H mutation i Actin
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Aip1p Dynamics Are Altered by the R256H Mutation in Actin

Aip1p Dynamics ændres med R256H mutation i Actin

DOI:
10.3791/51551-v

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08:57 min

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July 30, 2014

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, , ,
1Department of Pediatrics, Roy J. and Lucille A. Carver College of Medicine,University of Iowa, 2Department of Biochemistry, Roy J. and Lucille A. Carver College of Medicine,University of Iowa

Chapters

  • 00:04Title
  • 01:42Transforming the Plasmid into Yeast Cells
  • 03:36Visualizing Aip1p-GFP Protein Movement Using Total Internal Reflection Fluorescence Microscopy
  • 06:57Results: Quantification of Fluorescently Tagged Aip1p Movement in Wild Type and Mutant Cells
  • 08:29Conclusion

Summary

자동 번역

자동 번역

Sygdomsfremkaldende mutationer i actin kan ændre cytoskelet funktion. Cytoskeletal dynamik kvantificeres gennem billeddannelse af fluorescens mærkede proteiner ved hjælp af total intern fluorescens mikroskopi. Som et eksempel har cytoskeletprotein, Aip1p, ændret lokalisering og bevægelse i celler, der udtrykker mutant actin isoform R256H.

Tags

ActinCytoskeletal DynamicsTIRF MicroscopyR256H MutationAip1pActin DepolymerizationLive Cell ImagingCytoskeletal FunctionRegulatory InteractionsProtein Function

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