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Chapters
- 00:05Title
- 01:23Protein Pulldowns: Preparation of Transiently Transfected Cells
- 02:50Equilibration of the HaloLink Resin
- 04:01Binding and Washing of Fusion Complexes
- 06:21SDS Elution for Denaturing Gels, Western Blots, or Mass Spectrometry
- 07:07Cellular Imaging of Fluorescently Labeled Fusion Proteins Using a Cytoplasmic and Nuclear Permeable Ligand
- 08:48Results: Using HaloTag Technology to Determine Protein Interactions and Localization
- 10:47Conclusion
HaloTag teknologi er en multifunktionel teknologi, som har vist stor succes i isolering af både små og store protein-komplekser fra pattedyrceller. Her vil vi fremhæve fordelene ved denne teknologi i forhold til de eksisterende alternativer og vise sin nytte at studere mange aspekter af protein funktion inde eukaryote celler.
