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QPCR هو أسلوب حساس وسريع للكشف عن الحمض النووي من Cytomegaloviral في الفورمالين الثابتة، جزءا لا يتجزأ من البارافين-الأنسجة خزعة
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JoVE 신문 생물학
qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue
DOI:

08:08 min

July 09, 2014

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Chapters

  • 00:05Title
  • 01:24Processing FFPE GI Tissue Blocks
  • 03:17DNA Extraction from Tissue Scrolls
  • 04:30Quantitative PCR
  • 06:39qPCR Results on Control Tissues
  • 07:41Conclusion

Summary

자동 번역

This protocol describes qPCR detection of cytomegalovirus in formalin-fixed, paraffin-embedded biopsy tissue, which is rapid, sensitive, specific, and useful for interpreting equivocal hematoxylin and eosin or immunohistochemical staining patterns.

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