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1Department of Medical Microbiology, Immunology & Cell Biology,Southern Illinois University School of Medicine, 2Division of Orthopaedics and Rehabilitation, Department of Surgery,Southern Illinois University School of Medicine, 3Department of Electrical and Computer Engineering, Biomedical Engineering Program,Southern Illinois University Carbondale, 4University of Illinois at Springfield
Chapters
- 00:05Title
- 01:23Tissue Digestion and hACL Isolation
- 02:16Cellular Expansion, Freezing and Thawing
- 03:26Fabrication of Tissue Engineered 2-D Poly Lactic-co-glycolic Acid (PLAGA) Scaffold
- 04:22Scanning Electron Microscopy (SEM)
- 05:54Immunofluorescence Staining
- 07:01Results: Cellular Adhesion and Morphology Confirm Biocompatibility on Scaffold Surface
- 08:02Conclusion
For fremtidige programmer som en patch til at reparere partielle tårer af forreste korsbånd (ACL) blev humane ACL afledte celler isoleret fra væv opnået under rekonstruktive procedurer, ekspanderet in vitro og dyrket på manipuleret væv stillads. Cellulær adhæsion og morfologi blev derefter udført for at bekræfte biokompatibilitet på stillads overflade.
