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Flat Mount Forberedelse til observation og analyse af Zebrafisk Embryo Enheder Stained af Whole Mount<em> In situ</em> Hybridisering
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Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization

Flat Mount Forberedelse til observation og analyse af Zebrafisk Embryo Enheder Stained af Whole Mount<em> In situ</em> Hybridisering

DOI:
10.3791/51604-v

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06:36 min

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July 17, 2014

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, , , ,
1Department of Biological Sciences,University of Notre Dame

Chapters

  • 00:05Title
  • 01:37Dissection and Initial Deyolking of Embryo
  • 02:26Fine Deyolking of the Embryo
  • 03:18Mounting Embryo on a Glass Slide
  • 04:23Results: In situ Hybridization of Flat-mounted Embryos
  • 05:58Conclusion

Summary

자동 번역

자동 번역

Zebrafisk embryo er en fremragende model for udviklingsmæssige biologi forskning. Under embryogenese, zebrafisk udvikler sig med en æggeblomme masse, som præsenterer tre-dimensionelle udfordringer for prøve observation og analyse. Denne protokol beskriver, hvordan man opretter todimensionale flade mount præparater af hele mount in situ (ønske) farvet zebrafisk embryo prøver.

Tags

Zebrafish EmbryoDevelopmental StagesWhole Mount In Situ Hybridization (WISH)Flat Mount PreparationGene ExpressionEmbryonic Structures발생학Biomedical Research

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