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Flat Mount Forberedelse for observasjon og analyse av Sebrafisk Embryo prøver farget av Whole Mount<em> In situ</em> Hybridisering
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Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization

Flat Mount Forberedelse for observasjon og analyse av Sebrafisk Embryo prøver farget av Whole Mount<em> In situ</em> Hybridisering

DOI:
10.3791/51604-v

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06:36 min

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July 17, 2014

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, , , ,
1Department of Biological Sciences,University of Notre Dame

Chapters

  • 00:05Title
  • 01:37Dissection and Initial Deyolking of Embryo
  • 02:26Fine Deyolking of the Embryo
  • 03:18Mounting Embryo on a Glass Slide
  • 04:23Results: In situ Hybridization of Flat-mounted Embryos
  • 05:58Conclusion

Summary

자동 번역

자동 번역

Sebrafisk embryo er en utmerket modell for utviklingsbiologi forskning. Under embryogenesis, sebrafisk utvikle med en eggeplomme masse, som presenterer tredimensjonale utfordringer for prøve observasjon og analyse. Denne protokollen beskriver hvordan du oppretter todimensjonale flate montere preparater av hele fjellet in situ (skulle ønske) farget sebrafisk embryo prøver.

Tags

Keywords: Zebrafish EmbryoDevelopmental StagesWhole Mount In Situ Hybridization (WISH)Flat Mount PreparationGene ExpressionEmbryonic Structures발생학Biomedical Research

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