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Isolation of Neonatal Extrahepatic Cholangiocytes
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Isolation of Neonatal Extrahepatic Cholangiocytes

Isolation of Neonatal Extrahepatic Cholangiocytes

DOI:
10.3791/51621-v

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07:54 min

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June 05, 2014

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,
1Division of Gastroenterology, Hepatology, and Nutrition,The Children’s Hospital of Philadelphia, 2Department of Medicine,The Perelman School of Medicine at the University of Pennsylvania

Chapters

  • 00:05Title
  • 01:12Bile Duct Isolation
  • 02:59Embedding Tissues into Thick Collagen Gels
  • 04:19Isolating Primary Cholangiocytes
  • 07:05Characterizing Cultured Cholangiocytes
  • 07:24Conclusion

Summary

자동 번역

자동 번역

A technique to isolate cholangiocytes from the extrahepatic bile ducts of neonatal mice is described. The ducts are meticulously dissected, and then cells are isolated by outgrowth in thick collagen gels. This method provides a useful tool for studying extrahepatic bile duct development and pathology.

Tags

Neonatal Extrahepatic CholangiocytesBile DuctsIntrahepaticExtrahepaticCell IsolationCollagenCell CultureTrypsinizationCell PurificationBiliary Atresia

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