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新生儿肝外胆管的隔离
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JoVE 신문 생물학
Isolation of Neonatal Extrahepatic Cholangiocytes
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新生儿肝外胆管的隔离

DOI:
10.3791/51621-v

•

07:54 min

•

June 05, 2014

•

,
1Division of Gastroenterology, Hepatology, and Nutrition,The Children’s Hospital of Philadelphia, 2Department of Medicine,The Perelman School of Medicine at the University of Pennsylvania

Chapters

  • 00:05Title
  • 01:12Bile Duct Isolation
  • 02:59Embedding Tissues into Thick Collagen Gels
  • 04:19Isolating Primary Cholangiocytes
  • 07:05Characterizing Cultured Cholangiocytes
  • 07:24Conclusion

Summary

자동 번역

자동 번역

一种技术，从新生小鼠的肝外胆管胆管隔离描述。管道正在认真解剖，然后细胞被生长在厚厚的胶原凝胶中分离。该方法为研究肝外胆管发育和病理的有用工具。

Tags

Keywords: Neonatal Extrahepatic CholangiocytesBile DuctsIntrahepaticExtrahepaticCell IsolationCollagenCell CultureTrypsinizationCell PurificationBiliary Atresia

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