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Chapters
- 00:05Title
- 02:13Preparation of Attenuated Total Reflection Experiments on Bacteriorhodopsin
- 03:48Performing Transmission Experiments on Detergent-solubilized Channelrhodopsin-2
- 05:02Adjustment and Synchronization of the Exciting Laser
- 05:55Time-resolved Step-scan Preparations and Settings
- 07:48Results: Time-resolved Step-scan FTIR Experiments to Determine Protonation and Protein Conformational Changes
- 09:24Conclusion
Key steps of protein function, in particular backbone conformational changes and proton transfer reactions, often take place in the microsecond to millisecond time scale. These dynamical processes can be studied by time-resolved step-scan Fourier-transform infrared spectroscopy, in particular for proteins whose function is triggered by light.
