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Chapters
- 00:05Title
- 02:57Induction of Differentiation in Female Embryonic Stem Cells to Induce X Chromosome Inactivation
- 04:55Fixation of Differentiated ES Cells for Subsequent DNA-RNA FISH Analysis
- 05:33Labeling of DNA Probes for FISH Experiments
- 08:02Permeabilization, Pre-treatment and Hybridization of Fixed Cells for Combined DNA-RNA FISH
- 11:04Post-hybridization Washes and Antibody Mediated Detection of Probe
- 13:21Results: X Chromosome Inactivation in Differentiating Female ES Cells is Visualized by Combined DNA-RNA FISH
- 14:57Conclusion
Fluorescent in situ hybridization (FISH) allows the detection of nucleic acids in their native environment within cells. We here describe a protocol for the combined, simultaneous detection of RNA and DNA by means of FISH, which can be used to study X chromosome inactivation in mouse embryonic stem cells.
