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Chapters
- 00:05Title
- 02:57Induction of Differentiation in Female Embryonic Stem Cells to Induce X Chromosome Inactivation
- 04:55Fixation of Differentiated ES Cells for Subsequent DNA-RNA FISH Analysis
- 05:33Labeling of DNA Probes for FISH Experiments
- 08:02Permeabilization, Pre-treatment and Hybridization of Fixed Cells for Combined DNA-RNA FISH
- 11:04Post-hybridization Washes and Antibody Mediated Detection of Probe
- 13:21Results: X Chromosome Inactivation in Differentiating Female ES Cells is Visualized by Combined DNA-RNA FISH
- 14:57Conclusion
Fluoreszenz-in situ-Hybridisierung (FISH) erlaubt den Nachweis von Nukleinsäuren in ihrer natürlichen Umgebung innerhalb der Zellen. Wir beschreiben ein Protokoll für den kombinierten, gleichzeitigen Nachweis von RNA und DNA mit Hilfe von FISH, die verwendet werden können, um X-Chromosom Inaktivierung in embryonalen Maus-Stammzellen zu untersuchen.
