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Chapters
- 00:05Title
- 01:10Handling and Cocaine Exposure
- 02:27Dissection of the Nucleus Accumbens
- 03:47RNA Extraction
- 04:46qPCR Analysis
- 07:04Results: Transcription Dynamics in the Nucleus Accumbens after Cocaine Exposure
- 07:51Conclusion
This manuscript describes a protocol that applies comprehensive profiling for analysis of transcriptional programs induced in specific brain nuclei of rodents following behavioral paradigms. Herein, this approach is illustrated in the context of profiling genes induced in the nucleus accumbens (NAc) of mice following acute cocaine exposure, utilizing microfluidic qPCR arrays.
Tags
Keywords:
Transcription DynamicsBrain SamplesBehavioral ExperienceGene ExpressionMolecular NeuroscienceNeuropsychiatric DisordersTranscriptional ProgramsNucleus AccumbensCocaine ExperienceRNA PurificationReverse TranscriptionMicrofluidic ArraysQPCR AnalysisSingle CellsWhole-genome StudiesMicroarraysRNAseq
