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Chapters
- 00:05Title
- 01:12Transfection of PCF Parasites with the Fluorescent Reporter Construct
- 03:28Making Stabilates
- 04:24Thawing Frozen Stocks
- 05:25Cytometer Setup
- 06:17Counting Cells and Measure Fluorescence Using the Flow Cytometer
- 07:33Data Analysis
- 08:06Results: Monitoring Real-time Glycosome Dynamics in Live Parasites
- 09:20Conclusion
Glycosome dynamics in African trypanosomes are difficult to study by traditional cell biology techniques such as electron and fluorescence microscopy. As a means of observing dynamic organelle behavior, a fluorescent-organelle reporter system has been used in conjunction with flow cytometry to monitor real-time glycosome dynamics in live parasites.
