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प्राथमिक चूहा नवजात cardiomyocytes लाइव सेल इमेजिंग के adenoviral के बाद और Lentiviral पारगमन Confocal डिस्क माइक्रोस्कोपी स्पिनिंग का प्रयोग
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JoVE 신문 생물학
Live Cell Imaging of Primary Rat Neonatal Cardiomyocytes Following Adenoviral and Lentiviral Transduction Using Confocal Spinning Disk Microscopy
DOI:

13:36 min

June 24, 2014

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Chapters

  • 00:05Title
  • 02:10Isolation of Rat Neonatal Cardiomyocytes
  • 06:52Lentiviral Transduction
  • 07:55Adenoviral Transduction
  • 08:55Live Cell Imaging
  • 11:22Results: Confocal Spinning Disk Microscopy Enables Functional Studies of Proteins in Rat Neonatal Cardiomyocytes
  • 13:01Conclusion

Summary

자동 번역

This protocol describes a method of live cell imaging using primary rat neonatal cardiomyocytes following lentiviral and adenoviral transduction using confocal spinning disk microscopy. This enables detailed observations of cellular processes in living cardiomyocytes.

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