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Chapters
- 00:05Title
- 01:01Saliva Collection and Storage
- 01:46Initial Preparations, Cell Lysis and RNA, Protein and Lipid Removal
- 03:46Isolation and Purification of Genomic DNA
- 05:43Genomic DNA Characterization
- 06:35Conclusion
DNA extraction from saliva can provide a readily available source of high molecular weight DNA, with little to no degradation/fragmentation. This protocol provides optimized parameters for saliva collection/storage and DNA extraction to be of sufficient quality and quantity for downstream DNA assays with high quality requirements.
