This content is Free Access.
Chapters
- 00:05Title
- 01:2816S rRNA Gene Amplicon Library Preparation by the Fusion Method
- 03:21Amplicon Purification, Quantification, and Pooling
- 04:58Emulsion PCR and Sequencing
- 08:12Basic Sequence Data Analysis
- 09:15Results: Sequence Analysis of the 16S rRNA Gene
- 09:57Conclusion
Characterizing microbial community has been a longstanding goal in environmental microbiology. Next-generation sequencing methods now allow for the characterization of microbial communities at an unprecedented depth with minimal cost and labor. We detail here our approach to sequence bacterial 16S ribosomal RNA genes using a benchtop sequencer.
