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DNA的亲和纯化的芯片（DAP芯片）方法来确定目标基因的细菌双组分调控系统

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DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

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DNA的亲和纯化的芯片（DAP芯片）方法来确定目标基因的细菌双组分调控系统

DOI:

10.3791/51715-v

•

12:24 min

•

July 21, 2014

•

Lara Rajeev, Eric G. Luning, Aindrila Mukhopadhyay

¹Physical Biosciences Division,Lawrence Berkeley National Laboratory

Chapters

00:05Title
01:57Determine Gene Target for Response Regulator Using Electrophoretic Mobility Shift Assay
03:54Verify Target Enrichment after Genomic DNA-protein Binding
07:19DNA Labeling and Array Hybridization
10:11Results: DAP-chip Analysis of Gene Targets and Binding Sites for Response Regulators
11:51Conclusion

Summary

자동 번역

这个视频文章介绍了一种体外基于微阵列方法来确定两个组件系统响应监管机构的靶基因和结合位点。

Tags

DNA-affinity-purified Chip DAP-chip Bacterial Two Component Regulatory Systems Gene Targets Transcription Factors ChIP-chip Response Regulators In Vitro Microarray Affinity Purification Tiling Array Binding Site Motifs Desulfovibrio Vulgaris Hildenborough Sigma54-dependent Response Regulator

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