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Chapters
- 00:05Title
- 01:49Sequence Design and Synthesis
- 04:17Making the MPRA Library
- 08:33Transfection, Perturbation, and RNA Isolation
- 09:23Interpreting MPRA Results
- 10:16Conclusion
The genetic reporter assay is a well-established and powerful tool for dissecting the relationship between DNA sequences and their gene regulatory activities. Coupling candidate regulatory elements to reporter genes that carry identifying sequence tags enables massive parallelization of these assays.
